EZ DNA Methylation kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.	Always include fully methylated (in-vitro methylated DNA) and non-methylated controls (Leucocyte DNA, DNMT1/3 double KO DNA) during MSP

Upstream tips
For best results, the CT Conversion Reagent should be prepared freshly and used immediately following preparation.
Protocol tips
Always include fully methylated (in-vitro methylated DNA) and non-methylated controls (Leucocyte DNA, DNMT1/3 double KO DNA) during MSP

Publication protocol

Bisulfite sequencing validation
Genomic DNA from HSCs, CMPs, and ERYs was bisulfite-converted with the EZ DNA Methylation-Direct kit (Zymo Research). Briefly, 500 ng of DNA was converted according to a standard protocol. The converted DNA was PCR-amplified with bisulfite primers designed using MethPrimer (Li and Dahiya 2002). The primer sequences are listed in Supplemental Table S5. PCR-amplified bisulfite products were cloned using the TOPO TA Cloning kit (Invitrogen) and sequenced. Bisulfite sequences were analyzed with the Quantification Tool for Methylation Analysis (Kumaki et al. 2008).

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Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA methylation profiling Whole genome profiling - mouse hematopoietic stem cells using EZ DNA Methylation kit from Zymo Research.

Manufacturer protocol

Download the product protocol from Zymo Research for EZ DNA Methylation kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA methylation profiling Whole genome profiling - mouse hematopoietic stem cells using EZ DNA Methylation kit from Zymo Research. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.