REPLI-g Mitochondrial DNA Kit (25)

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Publication protocol

RCA-based mtDNA isothermal amplification: With plasma or extracted total DNA as template, mtDNA was amplified using a REPLI-g mitochondrial DNA kit (Qiagen) in strict accordance with the manufacturer’s recommendations. According to Qiagen’s manufacturer’s instruction and information, there is no risk of lack of mitochondrial DNA amplification due to a change in the primer binding site (i.e., caused by a deletion). The reason being the use of several primers in the amplification reaction with the REPLI-g Mitochondrial DNA Kit (as described on the company’s webpage, https://www.qiagen.com/).
Amplified mtDNA was treated with EcoRI (Takara, Kyoto, Japan), and the digested mtDNA was subjected to electrophoresis to assess if amplification was successful (Supplementary Fig. 1).

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