EpiTect Whole Bisulfitome Kit (25)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Perfor sodium bisulfite modification of genomic DNA using an EpiTect Bisulfite kit (Qiagen)	Follow manufacturer's instructions

Upstream tips
Perfor sodium bisulfite modification of genomic DNA using an EpiTect Bisulfite kit (Qiagen)
Protocol tips
Follow manufacturer's instructions

Publication protocol

Whole genome amplification of bisulfite-modified DNA: WGA of bisulfite-modified DNA was performed using EpiTect Whole Bisulfitome kit (Qiagen), according to the manufacturer’s instructions. In brief, bisulfite-modified DNA was amplified with a reaction buffer containing phi29 DNA polymerase at 28 °C for 8 h. WGA included three independent experiments. In each experiment, the sample set contained 50 or 10 ng of bisulfite-modified DNA and DDW as the negative control. In performing WGA, UV-irradiation of all the equipment successfully suppressed nonspecific amplification from negative controls [18] (data not shown).

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Manufacturer protocol

Download the product protocol from Qiagen for EpiTect Whole Bisulfitome Kit (25) below.

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