EpiTect Fast FFPE Bisulfite Kit (50)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Stop the bisulfite reaction with 50 μl NaOH (5 M)

Protocol tips
Stop the bisulfite reaction with 50 μl NaOH (5 M)

Publication protocol

Bisulfite Conversion: Bisulfite conversion and subsequent purification was performed according to the respective kit protocols. The following kits were applied: EpiTect Bisulfite Kit (Catalogue No. 59104), EpiTect Fast DNA Bisulfite Kit (Catalogue No. 59824), EpiTect Fast FFPE Bisulfite Kit (Catalogue No. 59844, Qiagen GmbH, Hilden, Germany), EZ DNA Methylation-Direct Kit (Catalogue No. D5020), EZ DNA Methylation-Gold Kit (Catalogue No. D5005), EZ DNA Methylation-Lightning Kit (Catalogue No. D5030, Zymo Research, Inc., Irvine, CA, USA), innuCONVERT Bisulfite Basic Kit (Catalogue No. 845-IC-1000040), innuCONVERT Bisulfite All-In-One Kit (Catalogue No. 845-IC-2000040), innuCONVERT Bisulfite Body Fluids Kit (Catalogue No. 845-IC-3000040, Analytik Jena AG, Jena, Germany).
For the analysis of inappropriate conversion 1 μmol T5 MeCT5 oligonucleotides were applied to each bisulfite reaction. The bisulfite reactions were stopped with 50 μl NaOH (5 M) without subsequent purification.

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