QIAamp MinElute Virus Spin Kit (50)

Protocol tips

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	Follow manufacturer’s instructions

Protocol tips
Follow manufacturer’s instructions

Publication protocol

DNA extraction: For the IsoQuick protocol, 100 μl of CSF were extracted, and viral DNA was resuspended in 25 μl of RNase-free water as previously published (Smalling et al., 2002, Tang et al., 1998). For the MinElute protocol, viral DNA was extracted by the MinElute spin kit according to the manufacturer's instructions. In brief, 25 μl of protease was added to 100 μl of CSF; a Buffer AL/Carrier RNA mixture was made and added to the CSF/protease mixture. The sample mixture was incubated at 56 °C for 15 min, and 250 μl ethanol was added. The entire volume of sample was then transferred to a QIAamp MinElute column and centrifuged for 1 min at 6000 × g. A series of washes were performed and the total viral DNA was eluted into 55 μl of RNase-free water.

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Manufacturer protocol

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