QIAshredder (250)
DNA fragmentation Cell lines
Publication protocol
QIAshredding: One aliquot of each Trireagent or QIAgen DNA extract was fragmented using the QIAshredder according to the manufacturer’s instructions. The extracted DNA was pipetted repeatedly to maximize homogeneity, then 50-100μl was removed and loaded onto a QIAshredder spin column, which was spun at maximum speed for 3 minutes. The flow-through was resuspended and pipetted to a fresh labeled tube. DNA concentrations of the QIAshredded and unshredded DNA were measured by UV Spectrophotometry (NanoDrop).Full paper Login or join for free to view the full paper.
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