FlexiGene DNA Kit (250)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Use of 1hr incubation of samples of paraffin-embedded samples with FG2/Protease instead of 10 min (suggested by the manufacturer) to ensure complete tissue lysis

Protocol tips
Use of 1hr incubation of samples of paraffin-embedded samples with FG2/Protease instead of 10 min (suggested by the manufacturer) to ensure complete tissue lysis

Publication protocol

Further sample preparation was performed using five different kits from QIAGEN: FFPE DNA Tissue Kit (QIAGEN), EZ1 DNA Tissue Kit (QIAGEN), DNA Mini Kit (QIAGEN), DNA Blood Mini Kit (QIAGEN), and Flexi-Gene DNA Kit (QIAGEN). With the exception of the FlexiGene DNA Kit, a spin column had to be used for nucleic acid purification and proteinase K (QIAGEN) for the lysis of the tissue with all other kits. For the FlexiGene Kit, no columns were needed because the purification is solely done by centrifugation and the “QIAGEN protease” was used for the lysis of the tissue. The DNA extraction was performed according to the manufacturers’ instructions for all kits with only minor modifications.
These minor modifications comprised:
FFPE DNA Tissue Kit:
Starting the protocol with step 10 due to the already performed dewaxing of the samples
EZ1 DNA Tissue Kit:
Elution of the DNA in 50 μl instead of 200 μl to ensure identical initial DNA concentrations in all compared protocols
Incubation of the samples of the mice M3, M10, and M15, and the Sp-samples of all mice with proteinase K for 1 h 30 min because tissue lysis was not complete after one hour
DNA Mini Kit:
Elution of the DNA in 50 μl instead of 200 μl to ensure identical initial DNA concentrations in all compared protocols
Incubation of the samples of the mice M3, M10, and M15, and the Sp-samples of all mice with proteinase K for 1 h 15 min to ensure complete tissue lysis
FlexiGene DNA Kit:
One-hour incubation of samples of the mice M3, M10, M15, and Sp-samples of all mice with FG2/Protease instead of 10 min to ensure complete tissue lysis
The extracted DNA was diluted with a final volume of 50 μl bi-distilled water and stored in the freezer at –20 °C for further experiments.


Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Tissue - FFPE samples using FlexiGene DNA Kit (250) from Qiagen.

Manufacturer protocol

Download the product protocol from Qiagen for FlexiGene DNA Kit (250) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA isolation / purification Tissue - FFPE samples using FlexiGene DNA Kit (250) from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.