Gentra Puregene Mouse Tail Kit (4 g)

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Publication protocol

All animals used and the procedures performed in this study were approved by the National Human Genome Research Institute Animal Care and Use Committee. Cbfb-MYH11 conditional knock-in (Cbfb+/56M),23 Runx1-knockout (Runx1−/−),15 Runx1-lacZ-knock-in (Runx1+/lz),24 β-actin-Cre recombinase transgenic (Actb-Cre+)25 and Mx1- Cre recombinase transgenic (Mx1-Cre+) 26 mice have been described previously. All mice were maintained on a mixed C57BL/6;129/SvEv background and were genotyped by PCR with gene-specific primers (sequences available upon request) using tail-snip DNA prepared using Gentra Puregene Mouse Tail Kit (Qiagen, Venlo, the Netherlands). Polyinosine-polycytidylic acid (pIpC; InvivoGen, San Diego, CA, USA) was used to induce Cbfb-MYH11 expression as described previously.19 To accelerate leukemia development, mice were treated with ENU (N-ethyl-N-nitrosourea) as described previously.5 All mice were observed for leukemia development for 12 months.

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Manufacturer protocol

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