Gentra Puregene Mouse Tail Kit (4 g)

DNA isolation / purification Tissue - murine tail biopsies

Experiment
DNA isolation / purification Tissue - murine tail biopsies
Product
Gentra Puregene Mouse Tail Kit (4 g) from Qiagen
Manufacturer
Qiagen

Protocol tips

Publication protocol

All animals used and the procedures performed in this study were approved by the National Human Genome Research Institute Animal Care and Use Committee. Cbfb-MYH11 conditional knock-in (Cbfb+/56M),23 Runx1-knockout (Runx1−/−),15 Runx1-lacZ-knock-in (Runx1+/lz),24 β-actin-Cre recombinase transgenic (Actb-Cre+)25 and Mx1- Cre recombinase transgenic (Mx1-Cre+) 26 mice have been described previously. All mice were maintained on a mixed C57BL/6;129/SvEv background and were genotyped by PCR with gene-specific primers (sequences available upon request) using tail-snip DNA prepared using Gentra Puregene Mouse Tail Kit (Qiagen, Venlo, the Netherlands). Polyinosine-polycytidylic acid (pIpC; InvivoGen, San Diego, CA, USA) was used to induce Cbfb-MYH11 expression as described previously.19 To accelerate leukemia development, mice were treated with ENU (N-ethyl-N-nitrosourea) as described previously.5 All mice were observed for leukemia development for 12 months.

Full paper   Login or join for free to view the full paper.

Reviews

Gentra Puregene Mouse Tail Kit (4 g) from Qiagen has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

5 years ago

Author: Denmark

What DNA isolation kit would work for insect samples?

Hello everyone! I am currently using different DNA isolation kits to extract DNA from insects. Even though I am able to successfully extract DNA I would like to maximize the yield. Do you have any tips that might help me with that even if the kits are not specifically designed for insect samples?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Tissue - murine tail biopsies using Gentra Puregene Mouse Tail Kit (4 g) from Qiagen.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Qiagen for Gentra Puregene Mouse Tail Kit (4 g) below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA isolation / purification Tissue - murine tail biopsies using Gentra Puregene Mouse Tail Kit (4 g) from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms