QuantiTect Multiplex PCR NoROX Kit (1000)
PCR Multiplex PCR - Poultry DNA
Protocol tips
Publication protocol
Real-Time PCR: Reaction Set-Up: All real-time PCR assays were performed on a RotorGene 6000 (Qiagen, Hilden, Germany) according to the QuantiTect Multiplex PCR handbook (Qiagen, Hilden, Germany). The reaction was set up in 25 µL with primer and probe concentrations according to Table 2. The following cycler regime was used: 15 min at 95 °C, 35 cycles of 15 s at 95 °C, and 1 min at 60 °C, collecting the fluorescence signal at the end of each cycle.Full paper Login or join for free to view the full paper.
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Discussion
Discussion
5 years ago
Author:
R. Verma
Multiplex PCR and qPCR
I have been doing multiplex PCR and qPCR separately. Is there a way to combine them in a single reaction using Sybr Green?
Discussion
5 years ago
Author:
Roland Lafontaine
My multiplex PCR has decreased in sensitivity
Hello there! My multiplex PCR has decreased in sensitivity even though I have not changed any of the parameters. The materials are in good condition and the PCR machine is well calibrated. I have tested multiple samples but one of my targets has decreased in sensitivity.
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