QuantiTect Multiplex PCR NoROX Kit (1000)

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Publication protocol

Real-Time PCR: Reaction Set-Up: All real-time PCR assays were performed on a RotorGene 6000 (Qiagen, Hilden, Germany) according to the QuantiTect Multiplex PCR handbook (Qiagen, Hilden, Germany). The reaction was set up in 25 µL with primer and probe concentrations according to Table 2. The following cycler regime was used: 15 min at 95 °C, 35 cycles of 15 s at 95 °C, and 1 min at 60 °C, collecting the fluorescence signal at the end of each cycle.

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