QIAquick 96 PCR BioRobot Kit (4)

Protocol tips

Upstream tips	Protocol tips	Downstream tips

Publication protocol

Microarray assay: Defined 200- to 400-bp fragments of selected cDNAs (38) were generated by RT-PCR (Superscript II, Invitrogen, Groningen, The Netherlands) and were cloned into pGEM-T Vector (Promega, Mannheim, Germany), and the sequence was verified. Inserts were amplified by using vector sequence-derived primers with the sense primer carrying a 5'-amino modification. Amplified inserts were purified (Qiaquick 96 PCR BioRobot Kit, QIAGEN, Hilden, Germany), checked on an agarose gel, diluted to a concentration of 100 ng/μl, and finally spotted four times on treated glass slides with a customized ink jet spotter.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA gel extraction / PCR product purification Product size < 15Kb using QIAquick 96 PCR BioRobot Kit (4) from Qiagen.

Manufacturer protocol

Download the product protocol from Qiagen for QIAquick 96 PCR BioRobot Kit (4) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA gel extraction / PCR product purification Product size < 15Kb using QIAquick 96 PCR BioRobot Kit (4) from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.