QIAamp MinElute Virus Vacuum Kit (50)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Follow manufacturer’s instructions

Protocol tips
Follow manufacturer’s instructions

Publication protocol

RNA extraction: For the RNAzol B protocol, 100 μl of CSF or NPS was extracted as previously published (Smalling et al., 2002, Tang et al., 1999). For the MinElute protocol, viral RNA was extracted by the MinElute Vacuum kit according to the manufacturer's instructions. In brief, 100 μl CSF or 1.1 ml NPS-lysis buffer mixture was mixed with 75 μl of protease, and then 550 μl of a Buffer AL/Carrier RNA mixture was added. After incubating at 56 °C for 15 min, 600 μl of ethanol was added and the mixture was incubated for five more minutes. The entire volume of sample was then added to a spin column connected to a vacuum manifold. The sample was then drawn through the column to allow the nucleic acids to bind to the silica membrane. A series of washes were performed and viral RNA was eluted into 55 μl of sample diluent (Applied Biosystems, Branchburg, NJ) by centrifuging for 1 min at 12,000 × g.

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Viral - Viral CNS disease using QIAamp MinElute Virus Vacuum Kit (50) from Qiagen.

Manufacturer protocol

Download the product protocol from Qiagen for QIAamp MinElute Virus Vacuum Kit (50) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Viral - Viral CNS disease using QIAamp MinElute Virus Vacuum Kit (50) from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.