FastLane Cell Probe Kit (200)

RNA isolation / purification Cells - immortalized A549

Experiment
RNA isolation / purification Cells - immortalized A549
Product
FastLane Cell Probe Kit (200) from Qiagen
Manufacturer
Qiagen

Protocol tips

Publication protocol

Total RNA was prepared from cells grown in 384-well plates or 96-well plates with FastLane Cell Probe kit or FastLane Cell Probe Multiplex kit (Qiagen). RNA lysates were used in real-time one-step RT-qPCR reactions performed on a Lightcycler 480 instrument (Roche). Gene expression values were calculated using the comparative delta delta Ct (-􏰀􏰀Ct) method, using housekeeper gene Ct values and control-treated Ct for normalization as described previously (20). FAM MGB Assay Probes were obtained from Thermo Fisher Applied Biosystems: KRAS (Hs00364284 g1), FOSL1 (Hs00759776 S1), 18S rRNA (4352655), RAB5C (Hs00904926 g1), RNASEH1 (Hs 007702360 s1), KIF1A (Hs00987720 m1), KIF5C (Hs00189672 m1), RAB5A (Hs00702360 s1) and RAB5B (Hs00951468 M1). For dual-colour RT-qPCR reactions, 18S rRNA VIC (4318839) was used. For polymerase chain reaction (PCR) analysis of ASO dose response treatment, curves were plotted and data were analysed using non- linear regression and curves were fitted using log (inhibitor) versus response, variable slope (four parameters) equation using GraphPad Prism, curves were constrained between 0 and 1.



Full paper   Login or join for free to view the full paper.

Reviews

FastLane Cell Probe Kit (200) from Qiagen has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Discussion

5 years ago

Author: Switzerland

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

I am having trouble using the QiageN RNAeasy mini kit for CD34+ cells. Any tips?

Share your thoughts or question with experts in your field by adding a discussion!

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Cells - immortalized A549 using FastLane Cell Probe Kit (200) from Qiagen.

View full paper   Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Qiagen for FastLane Cell Probe Kit (200) below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Cells - immortalized A549 using FastLane Cell Probe Kit (200) from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Outsource your experiment

Fill out your contact details and receive price quotes in your Inbox

  Outsource experiment
Become shareholder Discussions About us Contact Privacy Terms