MasterPureTM-Yeast DNA Purification kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Include RNAse treatment for 15-20 min. - Use prewarmed TE buffer to elute the DNA

Downstream tips
- Include RNAse treatment for 15-20 min. - Use prewarmed TE buffer to elute the DNA

Publication protocol

DNA–RNA immunoprecipitation and deep sequencing (DRIP-seq)
Immunoprecipitation procedure was adapted from previous studies (Skourti-Stathaki et al. 2011; Ginno et al. 2012), and 1 × 108 yeast cells were used for each immunoprecipitation. Genomic DNA containing R-loops was purified using MasterPure Yeast DNA Purification Kit (Epicentre). DNA was fragmented with a cocktail of five restriction enzymes (HindIII, EcoRI, BsrGI, XbaI, and SspI) (New England Biolabs). Five micrograms S9.6 monoclonal antibody (gift from Dr. Stephen H. Leppla at NIH) or nonspecific mouse IgG was used for each immunoprecipitation. Input and precipitated DNA was made into sequencing libraries using DNA SMART ChIP-Seq Kit (Clontech).

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Manufacturer protocol

Download the product protocol from Epicentre for MasterPureTM-Yeast DNA Purification kit below.

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