QIAGEN Proteinase K (10 ml)

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Publication protocol

Morula cells were suspended in PCR tubes with blastocyst lysis buffer (BLB) containing 60 μg/ml yeast tRNA (Invitrogen, AM7119) and 125 μg/ml proteinase K (Qiagen, 1114886). To prepare crude genomic DNA, the PCR tubes were placed in a thermal cycler, incubated at 95°C for 10min and 56°C for 20min and then stored at 4 °C. Using a REPLI-g Mini Kit (Qiagen, 150025), the crude genomic DNA was PCR-amplified using KAPA HiFi HotStart DNA Polymerase (Roche) according to the manufacturer’s instructions. The resulting PCR amplicons carrying Illumina TruSeq HT dual indexes were subjected to 150-bp paired-end sequencing using an Illumina iSeq 100. The indel frequencies were cal- culated by MAUND, which is available at https://github. com/ibs-cge/maund.

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