YeaStar™ Genomic DNA Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Susceptibility to yeast lytic enzymes varies for different yeast species. If you see incomplete lysis, extend the first incubation time up to 2 hours or over 16 hours.

Protocol tips
Susceptibility to yeast lytic enzymes varies for different yeast species. If you see incomplete lysis, extend the first incubation time up to 2 hours or over 16 hours.

Publication protocol

Genome sequencing
The three strains - (1) (NRRL Y-1603 or ATCC 28485), (2) ‘WT’ (NRRL Y-11430 or ATCC 76273) and (3) GS115 (ATCC 20864) were grown overnight in YPD (BD Difco, Cat. # 242820). DNA was extracted using the YeaStar Genomic DNA Kit (Zymo Research, Cat. # D2002) and RNA using the YeaStar™ RNA Kit (Zymo Research, Cat. # R1002). The extracted DNA was sequenced on the Pacific Biosciences (PacBio) single molecule real-time (SMRT) platform. Genomic DNA was also sequenced on Illumina HiSeq2000 from both fragment and jumping libraries. Illumina fragment libraries were generated as previously described [] with the following modifications. For each sample, 100 ng of genomic DNA was sheared to 200 bp in size using a Covaris LE220 instrument (Covaris, MA) with the following parameters: temperature: 7–9 °C; duty cycle: 20 %; intensity: 5; cycles per burst: 200; time: 90 s; shearing tubes: Crimp Cap microTUBES with AFA fibers Covaris, MA). DNA fragments were end repaired, 3′ adenylated, ligated with indexed Illumina sequencing adapter, and PCR enriched, as previously described [].

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Manufacturer protocol

Download the product protocol from Zymo Research for YeaStar™ Genomic DNA Kit below.

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