QIAamp DNA Microbiome Kit (50)

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	Follow manufacturer's instructions

Protocol tips
Follow manufacturer's instructions

Publication protocol

DNA extraction: DNA was extracted using 12 different commercial kits (kit number 4 and 5 were similar, but had different lot numbers) according to manufactures’ protocols: 1. BiOstic Bacteremia DNA Isolation Kit (MO BIO, lot no.: BC16C25), 2. Microbial DNA Isolation Kit (MO BIO, lot no.: U16E2), 3. PowerLyzer UltraClean Microbial DNA Isolation Kit (MO BIO, lot no.: PL16C29), 4. UltraClean Tissue & Cells DNA Isolation Kit (MO BIO, lot no.: U15I14), 5. UltraClean Tissue & Cells DNA Isolation Kit (MO BIO, lot no.: U16D18), 6. PowerSoil DNA Isolation kit (MO BIO, lot no.: PS16C29), 7. MasterPure yeast DNA purification kit (Epicentre, lot no.: 0020027874), 8. QIAamp DNA Investigator Kit (QIAGEN, lot no.: 154018987), 9. QIAamp DNA Microbiome Kit (QIAGEN, lot no.: 154026306), 10. PureLink Genomic DNA Kit (Invitrogen, lot no.: 17462207), 11. PureLink Microbiome DNA purification (Invitrogen, lot. No.: 1761498), 12. MolYsis Complete5 Ultra-deep Microbiome prep kit (Molzym, lot no.: S22qKG020025). These kits were chosen because they were applied in published skin microbiome studies and/or recommended by the manufacturer to be useful for skin microbiome analysis. The combinations of kits, locations and subjects are described in Fig. S1 and Table S1.

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Manufacturer protocol

Download the product protocol from Qiagen for QIAamp DNA Microbiome Kit (50) below.

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