QIAamp Fast DNA Stool Mini Kit (50)

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Publication protocol

DNA Extraction: DNA extraction was carried out for all stool samples with two extraction kits:
Z—ZR Fecal DNA Mini Prep (Zymo Research, Freiburg, Germany): An isolation was conducted in accordance with the standard manufacturer’s protocol.
Q—QIAamp FAST DNA Stool Mini Kit (Qiagen, Hilden, Germany): The isolation was performed in accordance with the special manufacturer’s protocol for DNA isolation from large volume of stool with few modifications. In the first step, 1 g of stool sample diluted in 10 mL of InhibitEX Buffer was vortexed vigorously in 50 mL centrifuge tube containing glass beads. In the first incubation, the suspension was heated at 95 °C (5 min), according to manufacturer’s recommendation for samples difficult to lyse. Second incubation was carried out at 70 °C for 20 min. In the last step, after adding ATE buffer, samples were left at room temperature for 5 min. Then, the isolates were kept in −20 °C, until further analysis.

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