QIAamp UCP Pathogen Mini Kit

Protocol tips

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	To increase DNA yields, elute extracted DNA with a small volume (30 μl) of recommended elution buffer

Protocol tips
To increase DNA yields, elute extracted DNA with a small volume (30 μl) of recommended elution buffer

Publication protocol

DNA extraction: All DNA extractions were performed with 3.0 ml of the original samples. Three DNA extraction methods were tested: QIAamp UCP PurePathogen Blood Kit (Catalogue 50112, Qiagen, Hilden, Germany) named kit3 in this study, QIAamp UCP Pathogen Mini Kit (Catalogue 50214, Qiagen, Hilden, Germany) named kit2, and QIAamp DNA Microbiome Kit (Catalogue 51704, Qiagen, Hilden, Germany) named kit1. To increase DNA yields, DNA extracted with all methods was eluted with relatively small volume (30 μl) of recommended elution buffer. DNA was isolated exactly as the manufacturer’s instructions. DNA concentration was measured by Qubit® 2.0 Fluorometer (Life Technologies, Invitrogen, USA).
As a negative control, the same procedure was used with sterile water; no PCR products were detected in any experiment, indicating lack of contamination of any of the reagents used.

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Manufacturer protocol

Download the product protocol from Qiagen for QIAamp UCP Pathogen Mini Kit below.

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