MasterPureTM-Yeast DNA Purification kit

DNA isolation / purification Yeast - Pichia pastoris

Experiment

DNA isolation / purification Yeast - Pichia pastoris

Product

MasterPureTM-Yeast DNA Purification kit from Epicentre

Manufacturer

Epicentre

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Tips Publication protocol Reviews Discussion Papers Manufacturer protocol Videos

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Include RNAse treatment for 15-20 min. - Use prewarmed TE buffer to elute the DNA

Downstream tips
- Include RNAse treatment for 15-20 min. - Use prewarmed TE buffer to elute the DNA

Publication protocol

Isolation of genomic DNA and PCR assays
For the extraction of genomic DNA (gDNA) from P. pastoris the MasterPure™ Yeast DNA Purification Kit from Epicentre (Madison, WI) was used according to the manufacturer’s protocol.

gDNA from relevant clones was used for different PCR assays.

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Discussion

5 years ago

Author: France

How to avoid genomic DNA contamination when isolating mitochondrial DNA?

Greetings! I am trying to isolate mitochondrial DNA from S. cerevisiae while avoiding contamination from genomic DNA. Any and all help is greatly appreciated.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Yeast - Pichia pastoris using MasterPureTM-Yeast DNA Purification kit from Epicentre.

Paper title

Non-canonical integration events in encountered during standard transformation analysed with genome sequencing

Full paper

Manufacturer protocol

Download the product protocol from Epicentre for MasterPureTM-Yeast DNA Purification kit below.

Download manufacturer protocol

Videos

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