Qproteome Cell Compartment Kit

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	Use of the kit for subcellular enrichment of total nuclear proteins

Protocol tips
Use of the kit for subcellular enrichment of total nuclear proteins

Publication protocol

Sample Preparation: The Monocyte-Derived Macrophage (MDM) Reference Spectral Library: Samples used to generate the MDM reference spectral library were subjected to subcellular enrichment using the Qproteome Nuclear Protein kit for soluble nuclear proteins or the Qproteome Cell Compartment kit for total nuclear proteins (Qiagen; Valencia, CA). For each nuclear enrichment procedure, samples were pooled equivalently using 25 μg of protein from each donor (n = 4) for each treatment condition. Each pooled sample was processed using filter-assisted sample preparation (FASP).18 Peptides were fractionated by isoelectric point by OFFGEL electrophoresis using pH 3–10 OFFGEL strips (Agilent, Santa Clara, CA).12 Fractionated peptides were cleaned and prepared for mass spectrometry using C18 spin-columns following manufacturer protocols (Thermo Fisher; Rockford, IL).

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