QIAamp DNA Mini Kit

DNA isolation / purification Yeast - Cryptococcus neoformans

Experiment
DNA isolation / purification Yeast - Cryptococcus neoformans
Product
QIAamp DNA Mini Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Downstream tips
- Include RNAse treatment for 15-20 min.
- Use prewarmed TE buffer to elute the DNA

Publication protocol

DNA extraction
Two hundred microliters of each previously processed clinical sample or yeast suspension were used for DNA extraction and purification. The QIAamp® DNA Mini kit (Qiagen, Hildenberg, Germany) was used with some modifications: the initial incubation with lysis buffer was performed at 65° C for one hour, followed by AL buffer incubation at 90 °C for 10 min and an additional incubation with recombinant lyticase (1UI/μl) at 37° C for 45 min. For filamentous fungal isolates, DNA extraction was performed using the phenol-chloroform method or a commercial kit with Genomic G-100 columns (Qiagen Inc., CA) (Sambrook et al., 2001).33 DNA extraction from whole blood was performed using a protocol described by Einsele et al. (1997)34 with some modifications. The relative concentrations of DNA extracted were determined using a NanoDrop ND2000 (Thermo Scientific).

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Discussion

Discussion

2 years ago

Author: France

How to avoid genomic DNA contamination when isolating mitochondrial DNA?

Greetings! I am trying to isolate mitochondrial DNA from S. cerevisiae while avoiding contamination from genomic DNA. Any and all help is greatly appreciated.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Yeast - Cryptococcus neoformans using QIAamp DNA Mini Kit from Qiagen.

Paper title
Validation and clinical application of a molecular method for the identification of Cryptococcus neoformans/Cryptococcus gattii complex DNA in human clinical specimens.
Full paper
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Manufacturer protocol

Download the product protocol from Qiagen for QIAamp DNA Mini Kit below.

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