QIAamp DNA Mini Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Include RNAse treatment for 15-20 min. - Use prewarmed TE buffer to elute the DNA

Downstream tips
- Include RNAse treatment for 15-20 min. - Use prewarmed TE buffer to elute the DNA

Publication protocol

DNA extraction
Two hundred microliters of each previously processed clinical sample or yeast suspension were used for DNA extraction and purification. The QIAamp® DNA Mini kit (Qiagen, Hildenberg, Germany) was used with some modifications: the initial incubation with lysis buffer was performed at 65° C for one hour, followed by AL buffer incubation at 90 °C for 10 min and an additional incubation with recombinant lyticase (1UI/μl) at 37° C for 45 min. For filamentous fungal isolates, DNA extraction was performed using the phenol-chloroform method or a commercial kit with Genomic G-100 columns (Qiagen Inc., CA) (Sambrook et al., 2001).33 DNA extraction from whole blood was performed using a protocol described by Einsele et al. (1997)34 with some modifications. The relative concentrations of DNA extracted were determined using a NanoDrop ND2000 (Thermo Scientific).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Yeast - Cryptococcus neoformans using QIAamp DNA Mini Kit from Qiagen.

Manufacturer protocol

Download the product protocol from Qiagen for QIAamp DNA Mini Kit below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing DNA isolation / purification Yeast - Cryptococcus neoformans using QIAamp DNA Mini Kit from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.