EpiTect Plus DNA Bisulfite Kit

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Publication protocol

Un-methylated DNA and methylated DNA: The unmethylated human genomic DNA used was EpiScope Unmethylated HCT116 DKO gDNA (Takara Bio), DNA derived from double knock-out HCT116 cells (DNMT1 and DNMT3B knocked out). The methylated human genomic DNA was EpiScope Methylated HCT116 gDNA (Takara Bio), made by high-degree methylation of un-methylated DNA using CpG methylase.
2 µg of each genomic DNA sample was bisulfite-converted using an EpiTect Plus DNA Bisulfite Kit (Qiagen) and purified. Various mixtures of bisulfited unmethylated DNA and bisulfited methylated DNA were made to produce a calibration curve for methylation rates of 100%, 75%, 50%, 25%, 5%, and 0%.

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