AllPrep PowerViral DNA/RNA Kit (50)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Do not perform optional Steps 3–7 of Qiagen protocol for bead beating Between Steps 13 and 14 of the Qiagen protocol perform the DNase protocol according to the manufacturer’s instructions

Protocol tips
Do not perform optional Steps 3–7 of Qiagen protocol for bead beating Between Steps 13 and 14 of the Qiagen protocol perform the DNase protocol according to the manufacturer’s instructions

Publication protocol

Extraction and DNA removal: If frozen, remove the concentrate and allow to thaw at room temperature.
Centrifuge the thawed concentrate for 1 minute at 12,000 x g.
Remove 200 µL of the supernatant and add 4 µL of TV, 5 log(copies/reaction), to each sample.
Perform extractions in duplicate using Qiagen’s AllPrep PowerViral kit according to the manufacturer’s instructions.
Do not perform optional Steps 3–7 of Qiagen protocol for bead beating.
Between Steps 13 and 14 of the Qiagen protocol perform the DNase protocol according to the manufacturer’s instructions.

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Manufacturer protocol

Download the product protocol from Qiagen for AllPrep PowerViral DNA/RNA Kit (50) below.

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