MagAttract PowerMicrobiome DNA/RNA EP

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Publication protocol

Genomic DNA was isolated from cultures grown in lysogeny broth overnight at 37°C with shaking. DNA was extracted in a 96-well format from 100 μl of sample using the MagAttract PowerMicrobiome DNA/RNA kit (Qiagen, Hilden, Germany) automated on a Hamilton Microlab STAR robotic platform. Bead disruption was conducted on a TissueLyser II (20 Hz for 20 min) instrument in a 96-deep-well plate in the presence of 200 μl phenol-chloroform. Genomic DNA was eluted in 90 μl of water after magnetic bead cleanup. The resulting genomic DNA was quantified with PicoGreen. The sequencing libraries were generated with the KAPA HyperPrep kit (catalog number KK8504) and sequenced on the Illumina NovaSeq 6000 system using a 150-bp × 2 paired-end kit.

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Manufacturer protocol

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