MagAttract PowerSoil DNA KF Kit

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Publication protocol

Method 3: Qiagen MagAttract PowerSoil DNA KF Kit (Catalog #27000-4-KF): Microbial DNA extraction was performed using the Earth Microbiome Protocol (Marotz et al., 2017) with a modification to the beadbeating step as described above. A step-by-step description of this protocol is outlined in https://www.protocols.io/view/earth-microbiome-project-emp-high-throughput-htp-d-pdmdi46. Briefly, PowerBead Solution, RNase A, and SL Solution (a lysis butter) is added to each beadbeating tube followed by a heating step at 65°C for 10 min, beadbeating, transfer of the supernatant to 96-well plates for subsequent binding, washing, and elution steps on the KingFisher Flex.

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