DNeasy PowerClean Pro Cleanup Kit (50)

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	Follow manufacturer's instructions

Protocol tips
Follow manufacturer's instructions

Publication protocol

Since strong PCR inhibition was observed in some field samples collected in the spring of 2016 (see the Results section for detail), we tested (1) the purification of the extracted DNA using either Zymo OneStep PCR Inhibitor Removal kit (Zymo Research, Irvine, CA, USA) or DNeasy PowerClean Pro Cleanup Kit (Qiagen, Hilden, Germany) following the manufactures’ instruction, (2) the addition of bovine serum albumin (BSA) to reaction mixture of qPCR assays and (3) the use of qPCR master mix specialized for environmental samples (TaqMan Environmental Master Mix 2.0; Applied Biosystems, Foster City, CA, USA), to achieve an inhibition-free assay condition. By combining these tests, the assay protocol was optimized for the natural seawater samples from Otsuchi Bay, which was then applied for the field samples collected in 2017 and 2018.

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Manufacturer protocol

Download the product protocol from Qiagen for DNeasy PowerClean Pro Cleanup Kit (50) below.

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