PowerSoil® DNA isolation - DNeasy PowerSoil Pro Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Do not use bleach to clean the inside of the PowerVac™ Manifold or to rinse the PowerVac™ Mini Spin Filter Adapters when attached to the manifold.

Upstream tips
Do not use bleach to clean the inside of the PowerVac™ Manifold or to rinse the PowerVac™ Mini Spin Filter Adapters when attached to the manifold.

Publication protocol

DNA extraction
Four extraction techniques were performed in triplicate on the 200 μL aliquots of both the pooled plaque and pooled saliva homogenates. Three commonly used commercial DNA extraction kits were used as per manufacturer’s instructions, namely the MoBio PowerSoil DNA Isolation Kit (M), Qiagen QIAamp DNA Mini Kit (Q) and the Zymo Bacterial/Fungal DNA Mini Prep (Z) (). Additionally, a previously described phenol:chloroform-based method for DNA isolation from saliva was used (P) []. Mechanical cell rupture was performed using a Qiagen TissueLyser II at 30 Hz for 2 x 50 s, as applicable. For each method, an extraction blank (PCR-grade water) was used to ascertain potential kit and/or reagent contamination.

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Papers

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Manufacturer protocol

Download the product protocol from Qiagen for PowerSoil® DNA isolation - DNeasy PowerSoil Pro Kit below.

Download manufacturer protocol

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