QIAxcel DNA Fast Analysis Kit (3000)

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Publication protocol

Results of PCRs were evaluated by capillary electrophoresis with QIAxcel (Qiagen) using the QIAxcel DNA Fast Analysis kit, alignment markers (DNA QX Alignment Marker 15 bp/3 kb) and marker sizes (QX DNA Size Marker 50–3000 bp). Amplified samples were purified using EXOSAP-it® (USB® Products AffyInc., Ohio, USA), according to the manufacturer’s instructions, and sequenced in both directions by Macrogen Inc. (Amsterdam, The Netherlands). Sequences were assembled using the SeqMan Pro software, edited with the EditSeq software (Lasergene, DNASTAR, Madison WI, USA) and compared with available sequences using BLAST.

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