QIAxcel DNA High Resolution Kit (1200)

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Publication protocol

QIAxcel Procedure: In both countries, PCRs were performed according to an optimized PCR protocol using primers as described by Supply et al. (2006) for a 24-loci panel. PCR products were uploaded in the QIAxcel instrument for separation of fragments and allele designation by electrophoresis under controlled conditions as per manufacturer’s instructions. QIAxcel DNA High Resolution Kit (1200) containing the QIAxcel DNA High Resolution Gel Cartridge was used for the study in both countries. A positive control (H37Rv) was included in each PCR and QIAxcel run. QX Alignment Marker (15 bp–5 kb) and QX DNA Size Marker (100 bp–2.5 kb) were included in every QIAxcel run. Allele calling was performed by using QIAxcel ScreenGel software. Results were entered into an Excel spreadsheet and analyzed further for accuracy and concordance of results.

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