GeneRead Size Selection Kit (50)
Whole Genome Amplification NGS library purification
Publication protocol
Sequencing: For DNA fragmentation, we used 2 μg genomic DNA or WGA DNA (without subsequent purification of WGA DNA) and the Covaris® S220 instrument. After fragmentation, DNA was purified using the MinElute® PCR Purification Kit (QIAGEN) according to the protocol in the kit handbook. Library preparation was performed using either the GeneRead™ Library Prep Kit (I) (QIAGEN) according to the manufacturer’s instructions. The NGS library was purified using the GeneRead Size Selection Kit (QIAGEN) as per instructions in the kit handbook. We performed 150 bp paired-end sequencing on an Illumina® MiSeq® instrument.Full paper Login or join for free to view the full paper.
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