AllPrep DNA/RNA/Protein Mini Kit (50)

Protocol tips

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	Follow manufacturer’s instructions

Protocol tips
Follow manufacturer’s instructions

Publication protocol

Cell Lysis Preparation for the Subsequent Extractions. Time for Completion: 0:15 Hours per Batch of 12 Samples: The remaining procedures on the cell lysate are done in the BSC to avoid contamination. The following steps were taken from the manual of the AllPrep DNA/RNA/Protein Mini Kit (step 4 on page 31 to step 24, as well as Appendix E1–E4 on page 52; December 2014 version), RNeasy MinElute Cleanup Kit (Supplementary protocol RY38; November 2008 version), as well as RNase-Free DNase Set (June 2018 version). The combined flow of the protocol is stated below with some modifications.
42.Incubate the frozen cell lysate in a heater set at 30 °C until thawed.
43.Centrifuge the lysate for 3 min at 14,000× g at room temperature, and transfer the supernatant to an AllPrep DNA spin column placed in a 2 mL collection tube.
44.Close the lid gently, centrifuge for 30 s at 8000× g.
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Object name is mps-03-00061-i002.jpg. CRITICAL STEP: Up to 700 μL of sample can be loaded onto the AllPrep DNA spin column. Make sure that no liquid remains on the column membrane after centrifugation. If necessary, repeat the centrifugation until all liquid has passed through the membrane.
45.Transfer the AllPrep DNA spin column into a new 2 mL collection tube, and store at 4 °C for later DNA purification (starting at step 72).

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Manufacturer protocol

Download the product protocol from Qiagen for AllPrep DNA/RNA/Protein Mini Kit (50) below.

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