Allprotect Tissue Reagent (100 ml)

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Publication protocol

Many commercial RNA stabilization reagents are therefore available for producing purified RNA which can be stored at room temperature. In our study, the RNA quantity and quality produced using two RNA stabilization reagents, RNAlater RNA Stabilization Reagent and RNAprotect cell Reagent, were comparable to those achieved by frozen storage. However, AllProtect Tissue Reagent showed inferior RNA quantity and quality compared to frozen storage. AllProtect Tissue Reagent contains the stickiest solution among the three RNA stabilization reagents, and this may affect the separation of the exfoliated cells from the brush. Most cervico-vaginal cytology samples were acquired from patients during medical treatment. Under these conditions immediate delivery to the freezer may difficult, and room-temperature tissue storage with stabilizing reagents is important. Although RNA stabilization reagents are not a long-term solution (7 days at room temperature), the ability to store and freeze tissue in stabilization reagents may be valuable for gaining time until delivery to the freezer and may provide insurance against unexpected freezer failure or temperature fluctuations. Moreover, our novel method may contribute to molecular studies using other exfoliated cells, such as oral cytology.

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