mericon DNA Bacteria Plus Kit (50)
DNA isolation / purification Bacteria - Gram positive Clostridium botulinum
Protocol tips
Publication protocol
DNA extraction method: Three commercially available DNA extraction methods were tested on 71 naturally contaminated samples after enrichment. A decrease in the C. botulinum detection rate was observed when using InstaGene Matrix (Bio-Rad, Marne-la-Coquette, France) or Mericon DNA Bacteria Plus kit (Qiagen, Courtaboeuf, France) instead of the QiaAmp® DNA mini kit (Qiagen, Courtaboeuf, France): InstaGene Matrix (Bio-Rad, Marne-la-Coquette, France) allowed only 87.3% of detection and Mericon DNA Bacteria Plus kit (Qiagen, Courtaboeuf, France)) 69% of detection, while QiaAmp® DNA mini kit (Qiagen, Courtaboeuf, France) gave a 100% detection rate on the 71 samples examined. Considering this result, two other commercially available kits based on the same approach (enzymatic digestion and DNA purification on silica columns) were tested on spiked samples: the DNA Blood and Tissue kit and the Nucleospin Tissue (Macherey-Nagel, Hoerdt, France) and both gave a 100% detection. Based on these results, a DNA extraction method including an enzymatic digestion step and a DNA purification on silica column should be used for optimal detection of type C and D spores in avian liver.Full paper Login or join for free to view the full paper.
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Discussion
Discussion
5 years ago
Author:
How can I improve my DNA yield?
The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?
Discussion
5 years ago
Author:
Milena Alexeyeva
Tips on storing DNA templates?
Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?
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