DNeasy 96 Plant Kit (6)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Use of dried leaf	Follow manufacturer's instructions

Upstream tips
Use of dried leaf
Protocol tips
Follow manufacturer's instructions

Publication protocol

DNA isolation and amplification: For each sample, we isolated total genomic DNA from approximately 10 mg of dried leaf material using DNeasy 96 Plant Kits (QIAGEN) following manufacturer instructions. We performed DNA amplification with various annealing temperatures depending on the primers used (Table S1). In some instances the primer pairs available at the outset of the study did not work well for specific taxonomic groups. In particular, we designed new primers for both rbcL, and cox1 and several sets of taxonomically-specific primer sets for matK, rpoB and rpoC1 (Table S1). We did not test all the possible primer combinations on all samples, but rather designed primers for taxonomic groups as needed.



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Manufacturer protocol

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