MagAttract HMW DNA Kit (48)

DNA isolation / purification Bacteria - Gram negative Klebsiella

Experiment
DNA isolation / purification Bacteria - Gram negative Klebsiella
Product
MagAttract HMW DNA Kit (48) from Qiagen
Manufacturer
Qiagen

Protocol tips

Protocol tips
Follow manufacturer's instructions

Publication protocol

Klebsiella pneumoniae 234–12 was inoculated into 40 ml of brain-heart-infusion broth and incubated at 37 °C and shaking at 140 rpm for 15 hours, resulting in 4 × 109 bacterial cells per milliliter. This culture was aliquoted and the biomass was pelleted by centrifugation and stored at −20 °C prior to DNA extraction. Basic characteristics of the kits compared are listed in Table 1. Extraction costs per sample varied from € 1.10 to € 8.10, with salting-out kits being the least expensive (Table 1). Extractions were performed according to the manufacturers’ protocols, and DNA was eluted or redissolved, respectively, in nuclease free water. All kits required similar hands-on time (35–60 minutes for three samples), but the lengths of incubation periods and total completion times varied more widely (Table 1).

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Reviews

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Discussion

Discussion

5 years ago

Author: Israel

How can I improve my DNA yield?

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Discussion

5 years ago

Author: Milena Alexeyeva Russian Federation

Tips on storing DNA templates?

Hello there! I just started doing experiments on bacterial DNA and I would like your opinion on storing DNA templates. Which are the desired and most optimal conditions?

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Papers

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Manufacturer protocol

Download the product protocol from Qiagen for MagAttract HMW DNA Kit (48) below.

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