MagAttract HMW DNA Kit (48)

Protocol tips

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	Follow manufacturer's instructions

Protocol tips
Follow manufacturer's instructions

Publication protocol

Klebsiella pneumoniae 234–12 was inoculated into 40 ml of brain-heart-infusion broth and incubated at 37 °C and shaking at 140 rpm for 15 hours, resulting in 4 × 109 bacterial cells per milliliter. This culture was aliquoted and the biomass was pelleted by centrifugation and stored at −20 °C prior to DNA extraction. Basic characteristics of the kits compared are listed in Table 1. Extraction costs per sample varied from € 1.10 to € 8.10, with salting-out kits being the least expensive (Table 1). Extractions were performed according to the manufacturers’ protocols, and DNA was eluted or redissolved, respectively, in nuclease free water. All kits required similar hands-on time (35–60 minutes for three samples), but the lengths of incubation periods and total completion times varied more widely (Table 1).

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Manufacturer protocol

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