DyeEx 2.0 Spin Kit (250)

Protocol tips

Upstream tips	Protocol tips	Downstream tips

Publication protocol

We performed Sanger sequencing to validate mutations. Primers for PCR amplification were designed in Primer3 software (Supplementary Table 2), and PCR was performed using the GenPack PCR Core (Isogen, Russia). Resultant amplicons were cleaned using a Cleanup Standard Kit (Evrogen, Russia) and processed using the BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, USA), following the manufacturers’ protocols. Probes were purified using a DyeEx 2.0 Spin Kit (QIAGEN) and sequenced in a 3730xl DNA Analyzer (Applied Biosystems).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Removal of unincorporated dye terminators using DyeEx 2.0 Spin Kit (250) from Qiagen.

Manufacturer protocol

Download the product protocol from Qiagen for DyeEx 2.0 Spin Kit (250) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Removal of unincorporated dye terminators using DyeEx 2.0 Spin Kit (250) from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.