QIAamp DSP Virus Kit

DNA isolation / purification Tissue - blood / plasma

Experiment
DNA isolation / purification Tissue - blood / plasma
Product
QIAamp DSP Virus Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Publication protocol

Peripheral blood samples were collected from 18 healthy pregnant women (6th-14th week of pregnancy) and from 12 healthy unpregnant subjects. cftDNA was isolated using QIAamp Circulating Nucleic Acid Kit (CNAK) (Qiagen, Germany) and QIAamp DSP Virus Kit (DSPVK) (Qiagen, Germany) from 1 ml of plasma of each sample. Methylation-sensitive restriction was carried out to isolate cffDNA. Yield of cffDNA and cftDNA was quantified using digital PCR. To explain the difference in resulting efficacy of these two kits PCR inhibitors analysis was performed, as well as the optimal plasma input for DSPVK was investigated.

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Discussion

Discussion

5 years ago

Author: Denmark

What DNA isolation kit would work for insect samples?

Hello everyone! I am currently using different DNA isolation kits to extract DNA from insects. Even though I am able to successfully extract DNA I would like to maximize the yield. Do you have any tips that might help me with that even if the kits are not specifically designed for insect samples?

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Papers

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Manufacturer protocol

Download the product protocol from Qiagen for QIAamp DSP Virus Kit below.

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