HiSpeed Plasmid Midi Kit (25)
Plasmid Isolation E. coli
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Publication protocol
Plasmid construction: Human full-length YTHDF2 cDNA was cloned and inserted into the PiggyBac vector with a FLAG tag at the N-terminus. High-purity plasmids used for mammalian cell transfection and stable cell line preparation were prepared using the HiSpeed Plasmid Midi Kit (Qiagen). The WEE1 construct (Category Number RC209760) and empty vector control (Category Number PS100001) were ordered from OriGene.Full paper Login or join for free to view the full paper.
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