Oligotex mRNA Mini Kit (12)

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Publication protocol

Library construction: For rmRNA-seq library construction, we used Trizol for the isolation of total RNA from the cerebrum of a 10-week old adult male BALB/c mouse and the Ribo-minus Eukaryote kit (for RNA-seq, Invitrogen, cat.10837-08) for depleting ribosomal RNA. The yield is about 1 μg ribo-minus RNA out of 8 μg total RNA. For mRNA-seq library construction, we used a two-round protocol for polyA+ mRNA purification using the Oligotex mRNA Mini Kit (Qiagen, Cat ID70042).

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