RNAprotect Tissue Reagent (250 ml)

Protocol tips

Upstream tips	Protocol tips	Downstream tips

Publication protocol

RNA sequencing and analysis: C57BL/6J mice negative for the RD8 mutation were purchased from Charles River and analyzed at the age of 8 weeks. Total bulk RNA was extracted from directly into RNAprotect buffer (QIAGEN, Hilden, Germany) FACS‐sorted CD45+CD11b+Ly6C−Ly6G− retinal and brain microglia, CD45+CD11b+CD64+F4/80+Ly6C−Ly6G− cornea macrophages and CD45+CD11b+SScloCD115+Ly6Chi BM monocytes, according to the “Purification of total RNA from animal and human cells” protocol of the RNeasy Plus Micro Kit (QIAGEN). In brief, cells were stored and shipped in RNAprotect buffer at 2–8°C. After pelleting, the RNAprotect buffer was replaced by RLT Plus buffer (QIAGEN) and the samples were homogenized by vortexing for 30 s. Genomic DNA contamination was removed using gDNA Eliminator spin columns. Next ethanol was added and the samples were applied to RNeasy MinElute spin columns followed by several wash steps. Finally, total RNA was eluted in 12 μl of nuclease free water. Purity and integrity of the RNA were assessed on the Agilent 2100 Bioanalyzer with the RNA 6000 Pico LabChip reagent set (Agilent, Palo Alto, CA, USA).



Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA stabilization Sorted cells using RNAprotect Tissue Reagent (250 ml) from Qiagen.

Manufacturer protocol

Download the product protocol from Qiagen for RNAprotect Tissue Reagent (250 ml) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA stabilization Sorted cells using RNAprotect Tissue Reagent (250 ml) from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.