DNeasy UltraClean Microbial Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Include RNAse treatment for 15-20 min. - Use prewarmed TE buffer to elute the DNA

Downstream tips
- Include RNAse treatment for 15-20 min. - Use prewarmed TE buffer to elute the DNA

Publication protocol

DNA extraction.
DNA from each of the 99 colonizing isolates was extracted using the UltraClean microbial DNA isolation kit (Mo Bio Laboratories) by following the manufacturer's instructions. We used 5 U/μl of the enzyme Zymolyase (Zymo Research Corporation, Irvine, CA). The DNA concentration was adjusted to approximately 25 ng/μl for each sample.

Molecular characterization by automated rep-PCR typing system (DiversiLab).


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Manufacturer protocol

Download the product protocol from Qiagen for DNeasy UltraClean Microbial Kit below.

Download manufacturer protocol

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