DNeasy UltraClean Microbial Kit

DNA isolation / purification Yeast - Candida parapsilosis

Experiment
DNA isolation / purification Yeast - Candida parapsilosis
Product
DNeasy UltraClean Microbial Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Downstream tips
- Include RNAse treatment for 15-20 min.
- Use prewarmed TE buffer to elute the DNA

Publication protocol

DNA extraction.
DNA from each of the 99 colonizing isolates was extracted using the UltraClean microbial DNA isolation kit (Mo Bio Laboratories) by following the manufacturer's instructions. We used 5 U/μl of the enzyme Zymolyase (Zymo Research Corporation, Irvine, CA). The DNA concentration was adjusted to approximately 25 ng/μl for each sample.

Molecular characterization by automated rep-PCR typing system (DiversiLab).


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Discussion

Discussion

5 years ago

Author: France

How to avoid genomic DNA contamination when isolating mitochondrial DNA?

Greetings! I am trying to isolate mitochondrial DNA from S. cerevisiae while avoiding contamination from genomic DNA. Any and all help is greatly appreciated.

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing DNA isolation / purification Yeast - Candida parapsilosis using DNeasy UltraClean Microbial Kit from Qiagen.

Paper title
Microsatellite Genotyping Clarified Conspicuous Accumulation of at a Cardiothoracic Surgery Intensive Care Unit
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Manufacturer protocol

Download the product protocol from Qiagen for DNeasy UltraClean Microbial Kit below.

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Videos

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