QIAamp 96 Virus QIAcube HT Kit (5)
RNA isolation / purification Viral - SARS-CoV-2
Publication protocol
Nucleic acid extraction and complementary DNA (cDNA) synthesis: 200μl of pooled sample was extracted for viral RNA with the QIAamp 96 Virus QIAcube HT kit (Qiagen, Germany) on the QIAcube HT System (QIAGEN, Germany) according to manufacturer’s instructions. Purified nucleic acid was then immediately converted to cDNA by reverse transcription with random hexamers using the SensiFAST cDNA Synthesis Kit (Bioline Reagents Ltd., UK) according to manufacturer’s instructions. cDNA was used immediately in the rRT-PCR or stored at -20 ̊C.Full paper Login or join for free to view the full paper.
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Manufacturer protocol
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