RNeasy 96 Universal Tissue Kit (4)
RNA isolation / purification Tissue - Mouse Kidney
Publication protocol
mRNA Analyses: For mRNA analysis, fresh renal cortex samples were collected from UUO studies using a 2-mm-diameter biopsy punch (Integra, Plains- boro, NJ) and placed in RNALater solution (Life Technologies, Carls- bad, CA). Total RNA was isolated from homogenized cortex punches using the Qiagen RNeasy-96 Universal Tissue Kit (Qiagen, Valencia, CA) following the manufacturer’s protocol. Reverse transcription of RNA to cDNA was done using Bio-Rad iScript cDNA Synthesis kits (Bio-Rad, Hercules, CA). All genes of interest were targeted with TaqMan Assays-on-Demand Gene Expression primers with FAM dye-labeled MGB probes from Applied Biosystems (Foster City, CA). TaqMan Universal Master Mix II with UNG (Applied Biosys- tems) was used in PCR reactions. Real time PCR was performed using the Bio-Rad CFX384 Touch system (Bio-Rad).Full paper Login or join for free to view the full paper.
Reviews
RNeasy 96 Universal Tissue Kit (4) from Qiagen has not yet been reviewed for this experiment
We'd love it if you would be the first to write a review!
Discussion
Discussion
4 years ago
Author:
Paul G. Macon
RNA isolation from tissue
How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?
Discussion
5 years ago
Author:
Aaron Stege
Problem in phase separation after using serum/plasma kit
I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?
Papers
Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Mouse Kidney using RNeasy 96 Universal Tissue Kit (4) from Qiagen.
Manufacturer protocol
Download the product protocol from Qiagen for RNeasy 96 Universal Tissue Kit (4) below.
Download manufacturer protocol
Videos
Check out videos that might be relevant for performing RNA isolation / purification Tissue - Mouse Kidney using RNeasy 96 Universal Tissue Kit (4) from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.
We haven't found any additional videos for this experiment / product combination yet.
Outsource your experiment
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment