RNeasy PowerBiofilm Kit (50)

Protocol tips

Upstream tips	Protocol tips	Downstream tips

Publication protocol

Nucleic acid extraction and cDNA synthesis. Extraction of the DNA and RNA molecules was performed with DNeasy PowerBiofilm and RNeasy PowerBiofilm kits (Qiagen), respectively, as recommended by the manufacturer, with minor modifications. Cell lysis was performed in a FastPrep-24 5G instrument at 6.5 m/s for 40 s, and nucleic acids were eluted with 60 μl of nuclease-free water. The DNA and RNA concentrations were quantified fluorometrically with the Qubit dsDNA and RNA HS assay kits (Life Technologies). Subsequently, total RNA was treated with a Turbo DNA-free kit (Invitrogen) to remove the remaining DNA. To verify the complete removal of DNA, a PCR targeting the 16S rRNA gene was performed using 27f and 1492r primers, described elsewhere (62). RNA was purified by using an RNeasy MinElute cleanup kit (Qiagen) and converted to cDNA by using a SuperScript IV first-strand synthesis system (Invitrogen).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Biofilm using RNeasy PowerBiofilm Kit (50) from Qiagen.

Manufacturer protocol

Download the product protocol from Qiagen for RNeasy PowerBiofilm Kit (50) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing RNA isolation / purification Biofilm using RNeasy PowerBiofilm Kit (50) from Qiagen. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.