RNeasy PowerClean Pro Cleanup Kit (50)

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Publication protocol

Culture of tumor single cell suspensions: Tumor single cell suspensions were thawed and seeded in U-bottomed 96-well plates at seeding densities of 20 × 104 cells/ml or 100 × 104 cells/ml. The cells were cultured in IMDM (ThermoFisher Scientific, #21980065) supplemented with 10% heat-inactivated fetal bovine serum (Sigma-Aldrich) and 100 U/ml penicillin-streptomycin (ThermoFisher Scientific, #15140122) for 48 h at 37°C and 5% CO2. At the end of the culture, cell culture supernatants were collected and frozen, while RNA was isolated from cells using the RNeasy Plus Mini Kit (Qiagen, #74134) according to manufacturer’s protocol. Potential contamination by melanin was removed with RNeasy PowerClean Pro Cleanup Kit (Qiagen, #13997-50).



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